pH value

7.0 (22 g/l, H₂O, 30 °C) (after autoclaving)

Bulk density

560 kg/m3

Solubility

22.5 g/l

Storage class

10 - 13 Other liquids and solids

WGK

WGK 1 slightly hazardous to water

Disposal

3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage

Store at +15°C to +25°C.

Appearance (clearness)

clear

Appearance (colour)

yellowish to yellowish-brown

Solidification behaviour (2 hrs., 40 °C)

liquid

Solidification behaviour (4 hrs., 45 °C)

liquid

pH-value (25 °C)

6.8 - 7.2

Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Incubation: 72 ± 3 hours at 30 ± 1 °C; aerobic

Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))

Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))

Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))

Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))

Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))

Inoculum on reference medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016))

10 - 100

Inoculum on reference medium (Listeria monocytogenes ATCC 19118)

10 - 100

Inoculum on reference medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098))

10 - 100

Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032))

Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034))

Colony count (Escherichia coli ATCC 8739 (WDCM 00012))

Colony count (Escherichia coli ATCC 25922 (WDCM 00013))

Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003))

Colony count (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016))

Colony count (Listeria monocytogenes ATCC 19118)

Colony count (Lactobacillus acidophilus ATCC 4356 (WDCM 00098))

Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))

≥ 70 %

Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))

≥ 70 %

Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))

≥ 70 %

Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))

≥ 70 %

Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003))

≥ 70 %

Recovery on test medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016))

≥ 70 %

Recovery on test medium (Listeria monocytogenes ATCC 19118)

≥ 70 %

Recovery on test medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098))

≥ 70 %

Incubation: 24 hours at 35 - 37 °C; aerobic

Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032))

Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))

Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))

Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))

Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))

Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032))

Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034))

Colony count (Escherichia coli ATCC 8739 (WDCM 00012))

Colony count (Escherichia coli ATCC 25922 (WDCM 00013))

Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003))

Recovery (Staphylococcus aureus ATCC 6538 (WDCM 00032))

≥ 70 %

Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))

≥ 70 %

Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))

≥ 70 %

Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))

≥ 70 %

Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003))

≥ 70 %

Reference: Tryptic soy agar.
S.aureus, E.coli and B.subtilis tested with pour plate technic.
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Other strains in single determination with spiral platemethode.